5TX3

Structure of Maternal Embryonic Leucine Zipper Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	64.620, 78.670, 78.960
Unit cell angles	90.00, 110.86, 90.00

Refinement procedure

Resolution	47.901 - 2.900
R-factor	0.1891
Rwork	0.182
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.304
Data reduction software	HKL
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.901
High resolution limit [Å]	2.900
Number of reflections	16392
<I/σ(I)>	12
Completeness [%]	99.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000