5TVT
Structure of Maternal Embryonic Leucine Zipper Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.690, 68.650, 105.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.362 - 2.280 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.769 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.362 |
High resolution limit [Å] | 2.280 |
Rmerge | 0.094 |
Number of reflections | 19214 |
<I/σ(I)> | 12.54 |
Completeness [%] | 99.6 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 24% PEG3350, 0.1 M MgCl, 0.1M HEPES pH 7.0 |