5TVS
JMJD2A in complex with Ni(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.689, 101.140, 148.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.832 - 2.745 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tvr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.832 | 2.840 |
| High resolution limit [Å] | 2.745 | 2.750 |
| Number of reflections | 23062 | |
| <I/σ(I)> | 35.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 17.5% PEG-3350 and 0.1 M citrate pH 6.0 |
