5TVB

Structure of the TPR oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-14
Detector	RAYONIX MX-225
Wavelength(s)	0.97622
Spacegroup name	C 1 2 1
Unit cell lengths	74.355, 50.669, 94.656
Unit cell angles	90.00, 108.01, 90.00

Refinement procedure

Resolution	41.187 - 2.750
R-factor	0.2474
Rwork	0.241
R-free	0.30270
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.572
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.187	2.750
High resolution limit [Å]	2.750	2.700
Rmerge		0.256
Number of reflections	9404
<I/σ(I)>	39.36
Completeness [%]	99.2	98.9
Redundancy	15.1	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	299	0.1M HEPES 7.5, 30% PEG1000