5TUW
Crystal structure of Orange Carotenoid Protein with partial loss of 3'OH Echinenone chromophore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 |
Unit cell lengths | 82.947, 82.914, 87.438 |
Unit cell angles | 89.79, 89.91, 60.04 |
Refinement procedure
Resolution | 41.490 - 2.302 |
R-factor | 0.1859 |
Rwork | 0.185 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mg1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.012 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.102 | |
Number of reflections | 85419 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 96.0 | 89 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 mM sodium acetate (pH 4.5) 16% PEG 8000 2% glycerol. |