5TUV
Crystal structure of the E2F5-DP1-p107 ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.980, 57.340, 99.198 |
| Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
| Resolution | 49.266 - 2.900 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.26010 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.816 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.570 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.410 |
| Number of reflections | 15301 | |
| <I/σ(I)> | 10.6 | 5.5 |
| Completeness [%] | 99.0 | 99.2 |
| Redundancy | 10.6 | 4.5 |
| CC(1/2) | 0.990 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100mM Hepes pH 7.0, 7% PEG 5000, 5% 1-propanol, 2% 2-propanol |
