5TUK

Crystal structure of tetracycline destructase Tet(51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-05-21
Detector	RDI CMOS_8M
Wavelength(s)	1.000028
Spacegroup name	P 1 21 1
Unit cell lengths	83.490, 81.690, 127.130
Unit cell angles	90.00, 96.79, 90.00

Refinement procedure

Resolution	19.904 - 1.850
R-factor	0.1672
Rwork	0.167
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.224
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1616)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.850	1.850
Number of reflections	144467
<I/σ(I)>	11.93
Completeness [%]	99.7
Redundancy	3.77
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES, pH 6.0, 10% PEG6000