5TUH

Archaellum periplasmic stator protein FlaG from Sulfolobus acidocaldarius

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0714, 1.0448, 1.0718
Spacegroup name	C 2 2 21
Unit cell lengths	83.023, 100.316, 62.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.816 - 1.929
R-factor	0.1822
Rwork	0.181
R-free	0.21320
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.815
Data reduction software	XSCALE
Data scaling software	Aimless (0.5.8)
Phasing software	SOLVE
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.816	44.820	1.970
High resolution limit [Å]	1.929	9.050	1.930
Rmerge	0.095	0.032	0.976
Number of reflections	20112
<I/σ(I)>	14.9		2.1
Completeness [%]	100.0	98.2	99.6
Redundancy	7.2	6.1	7
CC(1/2)	0.999	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	288	13%PEG3350, 0.2M sodium malonate, pH5, 3% 1,4 dioxane