5TUC
Crystal Structure of the Sus TBC1D15 GAP Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 139.000, 139.000, 175.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.620 - 2.500 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.873 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.172 | 1.254 |
| Number of reflections | 35232 | |
| <I/σ(I)> | 22.2 | 4.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 39.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 50 mM MES sodium Salt (pH 6.5), 20% (w/v) PEG1000, 100 mM sodium chloride and 200 mM magnesium chloride |
