5TUB
Crystal Structure of the Shark TBC1D15 GAP Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 224.790, 149.740, 95.030 |
| Unit cell angles | 90.00, 108.93, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.850 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tuc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.984 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.141 | 1.165 |
| Number of reflections | 69398 | |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.35 M ammonium sulfate, 0.09 M BIS-TRIS propane (pH 7.0) and 0.01 M calcium chloride dehydrate |
