5TU7
Crystal structure of Staphylococcus epidermidis Aap G511-spacer-G513 (consensus G5-spacer-consensus G5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.200, 55.100, 185.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.812 - 3.340 |
R-factor | 0.2596 |
Rwork | 0.256 |
R-free | 0.30670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.551 |
Data reduction software | XDS (May 1, 2016) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.812 | 3.460 |
High resolution limit [Å] | 3.340 | 3.340 |
Rmerge | 0.230 | 0.840 |
Number of reflections | 6930 | |
<I/σ(I)> | 8.9 | 2.67 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M sodium acetate pH 5.0 0.25 M calcium chloride 24% PEG 6000 |