5TU6
PagF prenyltransferase with cyclic[INPYLYP] and DMSPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 65 |
| Unit cell lengths | 116.490, 116.490, 43.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.220 |
| R-factor | 0.18434 |
| Rwork | 0.181 |
| R-free | 0.23945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.200 |
| Number of reflections | 116928 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 93.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | 20-25% PEG 3350 20 mM Tris-HCl pH=7.5 200 mM NaCl |
