5TTY
PagF prenyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 |
| Unit cell lengths | 116.930, 116.930, 43.642 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.19995 |
| Rwork | 0.199 |
| R-free | 0.22493 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.612 |
| Number of reflections | 140616 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 20-25% PEG 3350 20 mM Tris-Hcl pH 7.5 200 mM NaCl |
