5TTH
Heterodimeric SpyCatcher/SpyTag-fused zebrafish TRAP1 in ATP/ADP-hybrid state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 91.4 |
Detector technology | CCD |
Collection date | 2016-04-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.086, 95.711, 126.558 |
Unit cell angles | 90.00, 134.59, 90.00 |
Refinement procedure
Resolution | 49.000 - 3.200 |
R-factor | 0.2299 |
Rwork | 0.228 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j0b |
RMSD bond length | 0.012 |
RMSD bond angle | 1.490 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 90.130 |
High resolution limit [Å] | 3.200 |
Number of reflections | 21244 |
<I/σ(I)> | 9.1 |
Completeness [%] | 83.0 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.19 M potassium acetate, 20-22% PEG3350, Benzamidine hydrochloride (5-25 mM) |