5TSN
Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.760, 81.760, 189.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.908 - 2.100 |
R-factor | 0.1765 |
Rwork | 0.174 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bso |
RMSD bond length | 0.006 |
RMSD bond angle | 0.863 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.830 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.106 | 0.617 |
Number of reflections | 38050 | |
<I/σ(I)> | 14.2 | 4.3 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 11.2 | 12 |
CC(1/2) | 0.997 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 14% PEG8000, 25% glycerol, 100 mM Tris-HCl, pH 7.0, 50 mM potassium chloride, 10 mM manganese chloride |