5TS5
Crystal structure of L-amino acid oxidase from Bothrops atrox
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.640, 123.920, 105.080 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 34.572 - 2.300 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1reo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.629 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.840 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.146 | 0.557 |
| Number of reflections | 80024 | |
| <I/σ(I)> | 6.4 | 1.7 |
| Completeness [%] | 96.5 | 93.4 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 295 | zinc sulfate heptahydrate, sodium cacodylate pH 5, polyethylene glycol monomethyl ether 550 |
