5TQC
Crystal structure of transport factor karyopherin-beta 2 in complex with the PY-NLS of ribosomal protein L4 (RpL4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 68.630, 130.700, 174.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.671 - 3.000 |
R-factor | 0.2096 |
Rwork | 0.208 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jlq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 32119 | |
<I/σ(I)> | 20.7 | 1.64 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13.2 | 13.7 |
CC(1/2) | 0.999 | 0.757 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 0.5 M Sodium citrate tribasic 0.1 M BIS-TRIS-propane pH 7.0 |