5TPW

Crystal structure of amino terminal domains of the NMDA receptor subunit GluN1 and GluN2A in complex with zinc at the GluN2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2825
Spacegroup name	P 21 21 21
Unit cell lengths	79.560, 118.401, 155.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.563 - 2.909
R-factor	0.2042
Rwork	0.200
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qel
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Rmerge	0.115
Number of reflections	55256
<I/σ(I)>	26.14
Completeness [%]	100.0
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.8M Ammonium sulfate 2.5% Isopropanol