5TPN
Crystal structure of RSV F in complex with human antibody hRSV90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-04-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0331 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.250, 148.250, 538.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.289 - 3.141 |
| R-factor | 0.2263 |
| Rwork | 0.224 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c6b 4q9q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.10.1_2155-000) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.289 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.280 |
| Number of reflections | 30527 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 75.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG, 400/200 mM MgCl2, 6H2O/100 mM HEPES pH 7.5 |
