5TO6

Structure of the TPR oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-04
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	76.790, 34.860, 113.470
Unit cell angles	90.00, 91.85, 90.00

Refinement procedure

Resolution	46.327 - 2.700
R-factor	0.2392
Rwork	0.237
R-free	0.28820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5to5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.327	2.740
High resolution limit [Å]	2.700	2.700
Rmerge	0.149	0.615
Number of reflections	20228
<I/σ(I)>	10.3	1.4
Completeness [%]	94.5	73.1
Redundancy	7	5.4
CC(1/2)	0.998	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	299	0.1M Tris (8.0), 150mM Sodium malonate, 29% PEG1000