5TO5

Structure of the TPR oligomerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-16
Detector	RAYONIX MX300-HS
Wavelength(s)	1.07812
Spacegroup name	C 1 2 1
Unit cell lengths	74.177, 51.551, 94.980
Unit cell angles	90.00, 107.83, 90.00

Refinement procedure

Resolution	21.411 - 2.500
R-factor	0.2318
Rwork	0.229
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.722
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	21.850
High resolution limit [Å]	2.180
Rmerge	0.079
Number of reflections	17251
<I/σ(I)>	15.8
Completeness [%]	96.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.25	295	16% PEG1.5K, 0.1M Bistris (6.25)