5TMH

Structure of Zika virus NS5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	277
Detector technology	CCD
Collection date	2016-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 2 21 21
Unit cell lengths	95.136, 136.478, 196.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.244 - 3.278
R-factor	0.2636
Rwork	0.262
R-free	0.29330
RMSD bond length	0.002
RMSD bond angle	0.543
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.278
Number of reflections	39593
<I/σ(I)>	5.3
Completeness [%]	99.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.7-0.9 M lithium sulfate, 0.1 M MES, pH 6-7