5TMF
Re-refinement of thermus thermophilus RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 235.090, 235.090, 250.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.800 - 3.000 |
| R-factor | 0.20306 |
| Rwork | 0.203 |
| R-free | 0.23389 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Refinement software | REFMAC (5.5.0109) |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | MES, MAGNESIUM FORMATE, PEG 8000,SPERMINE, PH 5.85 |
