5TKW
1.35 Angstrom Resolution Crystal Structure of a Pullulanase-specific Type II Secretion System Integral Cytoplasmic Membrane Protein GspL (N-terminal fragment; residues 1-237) from Klebsiella pneumoniae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.901, 81.579, 40.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 1.350 |
R-factor | 0.1478 |
Rwork | 0.146 |
R-free | 0.17629 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.490 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.069 | 0.733 |
Number of reflections | 46935 | |
<I/σ(I)> | 45.9 | 2.03 |
Completeness [%] | 99.4 | 95.4 |
Redundancy | 6.7 | 3.8 |
CC(1/2) | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Protein: 8.9 mg/ml, 0.5M Sodium chloride, 0.1M Tris HCl (pH 8.3);Screen: Classics II (A5), 2.0M Ammonium sulfate, 0.1M HEPES (pH 7.5);Cryo: 2.0M Ammonium sulfate, 25% Sucrose. |