5TKO
RNA heptamer duplex with one 2'-5'-linkage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.541, 52.941, 27.732 |
| Unit cell angles | 90.00, 95.95, 90.00 |
Refinement procedure
| Resolution | 27.580 - 1.520 |
| R-factor | 0.14744 |
| Rwork | 0.144 |
| R-free | 0.17727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u37 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.708 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.083 | |
| Number of reflections | 11869 | |
| <I/σ(I)> | 17.8 | 1.95 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Precipitant: 40 mM sodium cacodylate pH5.5, 10% (v/v) MPD, 20 mM hexamine cobalt (III) chloride, 20mM MgCl2 Reservoir: 35% (v/v) MPD |
