5TKN
Crystal structure of FBP aldolase from Toxoplasma gondii, Schiff base FBP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 109.100, 109.100, 54.671 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.275 - 1.920 |
| R-factor | 0.1544 |
| Rwork | 0.150 |
| R-free | 0.19880 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 4.940 | 1.820 |
| Rmerge | 0.069 | 0.054 | |
| Total number of observations | 188737 | ||
| Number of reflections | 22332 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 73.8 | 99.8 | 13.3 |
| Redundancy | 8.5 | 11.9 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 0.1M Sodium Acetate, 0.2M Lithium Sulfate, 3.5% PEG 8000, 10% PEG 1000 |
