5THS
Crystal Structure of G302A HDAC8 in complex with M344
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.180, 84.460, 94.630 |
| Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
| Resolution | 46.730 - 1.900 |
| R-factor | 0.1783 |
| Rwork | 0.177 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ewf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.520 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.141 |
| Number of reflections | 65162 |
| <I/σ(I)> | 9.8 |
| Completeness [%] | 100.0 |
| Redundancy | 7.3 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM imidazole (pH 7.0), 15% w/v PEG 35000, 4 mM TCEP |
