5THL

Crystal structure of the human tyrosyl-tRNA synthetase mutant G41R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2011-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 2
Unit cell lengths	73.846, 162.954, 35.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.989 - 1.600
R-factor	0.1858
Rwork	0.185
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n3l
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.077	0.052	0.470
Total number of observations	392235
Number of reflections	57743
<I/σ(I)>	11.4
Completeness [%]	99.4	99.2	93.5
Redundancy	6.8	6.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	ammonium sulfate, acetone, sodium phosphate