5TH1

Crystal structure of H10 hemagglutinin mutant (K158aA-Q226L-G228S) from Jiangxi-Donghu (2013) H10N8 influenza virus in complex with 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	64.404, 254.466, 70.649
Unit cell angles	90.00, 112.03, 90.00

Refinement procedure

Resolution	48.820 - 2.191
R-factor	0.192
Rwork	0.190
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tgo
RMSD bond length	0.016
RMSD bond angle	2.566
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.190	2.190
Number of reflections	105198
<I/σ(I)>	27	4.4
Completeness [%]	97.9
Redundancy	3.7	3.6
CC(1/2)		0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	0.2 M NaCl, 10% (w/v) PEG 8000, 0.1M CHES pH 9.5