5TGE

Thermus Phage P74-26 Large Terminase Nuclease Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	71.370, 71.370, 127.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.507 - 2.600
R-factor	0.2211
Rwork	0.220
R-free	0.24540
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.504
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX ((1.10_2155))
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	10480
<I/σ(I)>	23.3
Completeness [%]	98.0
Redundancy	28
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	20 mg/mL protein mixed 2:1 with buffer containing 0.23M sodium phosphate monobasic/potassium phosphate dibasic pH 6.2 and 2.5M sodium chloride, and 4 mM dTMP