5TG8

Crystal structure of H15 hemagglutinin from A/shearwater/WA/2576/1979 H15N9 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 3
Unit cell lengths	115.991, 115.991, 128.451
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.388 - 3.100
R-factor	0.2241
Rwork	0.222
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n5j
RMSD bond length	0.014
RMSD bond angle	2.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	34747
<I/σ(I)>	9.1	1.7
Completeness [%]	98.7
Redundancy	4.1	3.1
CC(1/2)		0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	20% (w/v) PEG 3500, 0.2M potassium nitrate pH 6.9.