5TFW

Crystal structure of 10E8 Fab light chain mutant2 against the MPER region of the HIV-1 Env, in complex with T117v2 epitope scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	110
Detector technology	CCD
Collection date	2015-07-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	C 1 2 1
Unit cell lengths	213.490, 63.410, 53.870
Unit cell angles	90.00, 95.79, 90.00

Refinement procedure

Resolution	47.235 - 2.168
R-factor	0.1721
Rwork	0.169
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6f 3lf6
RMSD bond length	0.007
RMSD bond angle	0.871
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XSCALE (VERSION November 3, 2014 BUILT=20141118)
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.235	2.220
High resolution limit [Å]	2.168	2.168
Rmerge		0.635
Number of reflections	36138
<I/σ(I)>	15.5	2.1
Completeness [%]	94.5	95.8
Redundancy	4.2	4
CC(1/2)	0.999	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	295.15	0.2 M Na thiocyanate, 20% PEG 3350, pH 6.9, 10% ethylene glycol