5TFL
Crystal Structure of Mouse Cadherin-23 EC7+8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97921 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 185.809, 185.809, 185.809 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 131.390 - 3.560 |
R-factor | 0.19385 |
Rwork | 0.192 |
R-free | 0.22143 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Cadherin-23 (5I8D) |
RMSD bond length | 0.013 |
RMSD bond angle | 1.365 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 131.390 | 3.610 |
High resolution limit [Å] | 3.560 | 3.560 |
Rmerge | 0.363 | 1.587 |
Number of reflections | 13716 | |
<I/σ(I)> | 20 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 35.5 | 23.7 |
CC(1/2) | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 277 | 0.1 M Sodium Acetate pH 4.7, 1.9 M Sodium Chloride, 30% PEG 400 |