5TET
TEV Cleaved Human ATP Citrate Lyase Bound to 4S Hydroxycitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.210, 83.510, 193.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.080 - 2.200 |
R-factor | 0.159 |
Rwork | 0.156 |
R-free | 0.21300 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.003 |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 53.090 |
High resolution limit [Å] | 2.200 |
Number of reflections | 45650 |
<I/σ(I)> | 14 |
Completeness [%] | 99.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 12.5% of P3350, 100 mM TrisHCL pH 7.5, 125 mM ammonium phosphate pH 7.5 |