5TDW
Set3 PHD finger in complex with histone H3K4me3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-02 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 25.870, 43.680, 85.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.530 - 1.700 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.19910 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.540 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 11089 |
| <I/σ(I)> | 14 |
| Completeness [%] | 99.9 |
| Redundancy | 26.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 273 | 20% PEG3350, 0.2 M sodium citrate |
