5TDL

Crystal structure of prefusion-stabilized bovine RSV fusion glycoprotein (single-chain DS2-v1 variant: strain 391-2 sc9 DS-Cav1 Q98C Q361C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 3 2
Unit cell lengths	172.072, 172.072, 172.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.558 - 3.500
R-factor	0.2572
Rwork	0.256
R-free	0.27200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.558	3.590
High resolution limit [Å]	3.500	3.500
Number of reflections	11445
<I/σ(I)>	9.6	2
Completeness [%]	99.5	100
Redundancy	10.4	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.9 M of potassium and sodium tartrate, 0.16 M of Lithium sulfate, 0.1 M ches