5TDC
Crystal structure of the human UBR-box domain from UBR1 in complex with monomethylated arginine peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9769 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.274, 49.036, 53.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.736 - 1.607 |
| R-factor | 0.1505 |
| Rwork | 0.149 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ny1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.736 | 1.630 |
| High resolution limit [Å] | 1.607 | 1.607 |
| Number of reflections | 16360 | |
| <I/σ(I)> | 13.26 | |
| Completeness [%] | 97.4 | 86 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.2 M (NH4)2SO4, 0.2 K/Na Tartrate |
