5TD9

Structure of Human Enolase 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 2 21 21
Unit cell lengths	68.319, 112.868, 119.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.510 - 2.318
R-factor	0.2018
Rwork	0.200
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zcw
RMSD bond length	0.006
RMSD bond angle	0.636
Data reduction software	HKL-2000 (708)
Data scaling software	HKL-2000 (708)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1-2155-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.310	6.270	2.310
Rmerge	0.110	0.057	0.391
Rmeas	0.117
Rpim	0.041
Total number of observations	327713
Number of reflections	40898
<I/σ(I)>	10
Completeness [%]	99.8	99.8	96.4
Redundancy	8	7.4	7.3
CC(1/2)		0.997	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM Bis-Tris, pH 6.5, 200 mM ammonium acetate, 20% w/v PEG3350, cryo-soak in solution containing 100 mM Bis-Tris, pH 6.5, 200 mM ammonium acetate, 35% w/v PEG3350