5TBH

Crystal structure of mouse CARM1 in complex with inhibitor LH1236

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	74.647, 98.529, 206.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 2.341
R-factor	0.1954
Rwork	0.194
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.006
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1980)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.260	2.400
High resolution limit [Å]	2.340	2.340
Rmerge	0.275	1.836
Number of reflections	64779
<I/σ(I)>	7.4	1.8
Completeness [%]	99.6	90.9
Redundancy	6.3	5.9
CC(1/2)	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris-HCl pH 8.0 100 mM PEG 2000 MME 19% NaCl 100 mM