5TBF
Crystal structure of SeMet derivatives of domain2 and domain 3 of RctB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-19 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.526, 105.526, 137.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.370 - 3.003 |
R-factor | 0.2223 |
Rwork | 0.220 |
R-free | 0.27830 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.859 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((dev-2376_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 18054 |
<I/σ(I)> | 19.68 |
Completeness [%] | 99.1 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 1.5M Ammonium Sulfate |