5TB8
Precatalytic ternary complex of Human DNA Polymerase Beta in closed conformation With Gapped DNA substrate incoming (-)3TC-TP and Mn2+.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.480, 81.400, 53.570 |
| Unit cell angles | 90.00, 110.28, 90.00 |
Refinement procedure
| Resolution | 46.410 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.433 |
| Number of reflections | 26398 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 98.2 | 97.6 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50 MM IMIDAZOLE, 350 MM SODIUM ACETATE, 17% PEG3350, PH 8.0 |
