5TAO
Haloferax volcanii Malate Synthase Lead(II) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 155.353, 155.353, 139.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.810 - 2.100 |
| R-factor | 0.18477 |
| Rwork | 0.183 |
| R-free | 0.21668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pug |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.916 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.810 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.103 | 0.099 | 0.374 |
| Number of reflections | 35791 | ||
| <I/σ(I)> | 13.2 | ||
| Completeness [%] | 99.5 | 96.3 | 100 |
| Redundancy | 3.7 | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | magnesium chloride, glyoxylate, Tris, KCl, ammonium acetate, PEG 4500, glycerol, sodium acetate trihydrate |
