5T9T

Protocadherin Gamma B2 extracellular cadherin domains 1-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	80.722, 118.067, 98.190
Unit cell angles	90.00, 103.91, 90.00

Refinement procedure

Resolution	39.751 - 3.500
R-factor	0.2151
Rwork	0.213
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szr
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.780
High resolution limit [Å]	3.500	3.500
Rmerge	0.369	1.176
Number of reflections	22663
<I/σ(I)>	4.8	1.6
Completeness [%]	99.9	100
Redundancy	3.8	3.8
CC(1/2)	0.957	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	295	8.3% (w/v) PEG8000, 16.7% (v/v) ethylene glycol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1 M Morpheus Buffer System 2 (Hepes/MOPS buffer; Molecular Dimensions)