5T96
Crystal structure of the infectious salmon anemia virus (ISAV) HE viral receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.480, 87.568, 95.534 |
| Unit cell angles | 97.14, 113.50, 114.68 |
Refinement procedure
| Resolution | 48.396 - 2.000 |
| R-factor | 0.1391 |
| Rwork | 0.139 |
| R-free | 0.18260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t9y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.400 | 48.400 | 2.030 |
| High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
| Rmerge | 0.057 | 0.027 | 0.397 |
| Rmeas | 0.067 | 0.032 | 0.461 |
| Rpim | 0.034 | 0.016 | 0.234 |
| Total number of observations | 528952 | 3160 | 26031 |
| Number of reflections | 138068 | ||
| <I/σ(I)> | 14.9 | 38.1 | 3.5 |
| Completeness [%] | 97.9 | 98.5 | 96.6 |
| Redundancy | 3.8 | 3.7 | 3.9 |
| CC(1/2) | 0.999 | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | [0.2 M] magnesium formate, 20% PEG 3350, w/ a [0.8 M] sodium phosphate, [0.8 M] potassium phosphate, [0.1 M] HEPES pH 7.5 solution as a 1:6 ratio drop additive |
