5T94

Crystal structure of Kap60 bound to yeast RCC1 (Prp20)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-16
Detector	RAYONIX MX325HE
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	91.050, 99.200, 125.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.894 - 2.631
R-factor	0.2188
Rwork	0.217
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4PVZ and 3OF7
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.720
High resolution limit [Å]	2.630	2.630
Rmerge		0.644
Number of reflections	33934
<I/σ(I)>	49.9	3.4
Completeness [%]	96.0	85.8
Redundancy	4.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Na cacodylate, 0.1 M NaCl and 1.1 M ammonium sulfate