5T85

Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidylglycerol (06:0 PG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2014-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	55.006, 67.287, 194.678
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.669 - 2.373
R-factor	0.1979
Rwork	0.195
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6f 3lf6
RMSD bond length	0.008
RMSD bond angle	1.107
Data reduction software	HKL-2000 (2.3.7)
Data scaling software	HKL-2000 (2.3.7)
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.669	2.400
High resolution limit [Å]	2.373	2.373
Rmerge		0.702
Number of reflections	28788
<I/σ(I)>	8.5	1.8
Completeness [%]	95.7	98.1
Redundancy	3.5	3.7
CC(1/2)	0.915	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293.15	50% PEG 200, 0.1 M Hepes pH 7.0