5T7H
Crystal structure of dimeric yeast iso-1-cytochrome C with CYMAL6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.351, 56.122, 56.127 |
| Unit cell angles | 75.65, 63.37, 63.36 |
Refinement procedure
| Resolution | 7.980 - 2.003 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mu8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.150 | 2.080 |
| High resolution limit [Å] | 2.003 | 2.003 |
| Rmerge | 0.035 | 0.304 |
| Number of reflections | 31532 | |
| <I/σ(I)> | 11.9 | 2.26 |
| Completeness [%] | 95.8 | 90.3 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 75% (NH4)2SO4, AN 86% (NH4)2SO4 AND 0.1 M TRIS RESERVOIR SOLUTION, AND 5.6 MM CYMAL-6., PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
