5T7B

Argonaute-2 - 5'-(E)-vinylphosphonate 2'-O-methyl-uridine modified mrTTR guide RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	63.207, 108.306, 68.821
Unit cell angles	90.00, 106.62, 90.00

Refinement procedure

Resolution	65.946 - 2.529
R-factor	0.1957
Rwork	0.194
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f3t
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.950	2.537
High resolution limit [Å]	2.529	2.529
Rmerge	0.065	0.655
Number of reflections	27742
<I/σ(I)>	14.2	2.2
Completeness [%]	93.0	98.9
Redundancy	3.2	3.3
CC(1/2)	0.998	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	100 mM Tris pH=9.0, 10% PEG 3350 (w/v), 8% 2-propanol (v/v), 0.12 M phenol