5T5W

Structure of an affinity matured lambda-IFN/IFN-lambdaR1/IL-10Rbeta receptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999975
Spacegroup name	P 31 2 1
Unit cell lengths	106.790, 106.790, 129.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.241 - 2.847
R-factor	0.2009
Rwork	0.198
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3og6 3lqm
RMSD bond length	0.005
RMSD bond angle	0.694
Data reduction software	XDS (Oct 15, 2015)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.241		2.920
High resolution limit [Å]	2.847	12.730	2.850
Rmerge	0.086	0.040	3.243
Number of reflections	20507
<I/σ(I)>	24.14	59.54	1.13
Completeness [%]	99.9	94.2	99.8
Redundancy	21		21
CC(1/2)	1.000	0.999	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	.2 M Ca acetate, .1 M Imidazole pH 8.0, 10% PEG8000, 3% sucrose