5T5A

Crystal Structure of the Twister Sister (TS) Ribozyme at 2.0 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91772
Spacegroup name	P 41 21 2
Unit cell lengths	39.304, 39.304, 228.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.160 - 2.000
R-factor	0.1721
Rwork	0.170
R-free	0.21710
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.987
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.10.1_2155: ???))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.160	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.074	0.376
Number of reflections	268378
<I/σ(I)>	28.95
Completeness [%]	97.0
Redundancy	21.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	289	magnesium acetate sodium cacodylate MPD